Cst atg5
WebNational Center for Biotechnology Information WebDec 1, 2024 · In order to further quantify the effect of Ce6-PDT on autophagy, autophagy-related protein Atg5 was measured using western blotting. As shown in Fig. 3B, a dose-dependent increase in Atg5 levels was observed in the Ce6-PDT cells compared to the control and irradiation-only cells. These data suggest that Ce6-PDT induces autophagy …
Cst atg5
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WebSignaling Technology (CST) allows the researcher to specifically inhibit Atg5 expression using RNA interfer-ence, a method whereby gene expression can be selectively silenced … WebIn cervical cancer cells, ATG5 knockdown resulted in attenuation of migration and invasion. The functional study showed that knockdown of ATG5 could reverse EMT process by P-ERK, P-NFκBp65, P-mTOR pathways, and so on. In conclusion, the present study implies that ATG5 was a major contributor to EMT regulation and poor prognosis in cervical cancer.
WebThe ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in … WebSimilar to the in vitro data, endothelial-specific atg5 knockout mice (Atg5 Endo; Cdh5-Cre Atg5 flox/flox mice) displayed both EndMT-associated kidney and heart fibrosis when compared to littermate controls. The plasma level of IL6 was higher in Atg5 Endo compared to that of control mice, and fibrosis was accelerated in Atg5 Endo treated with a ...
WebCell Signaling Technology's Cathepsin B (D1C7Y) XP ® Rabbit mAb is a Rabbit monoclonal antibody. The Cathepsin B (D1C7Y) XP ® Rabbit mAb Antibody was generated using Cathepsin B, and CTSB as the antigen. It reacts with Human, Mouse, and Rat. This antibody has been shown to work in applications such as: Flow Cytometry, … WebHere, using mice with genetic ablation of the essential Atg5 (autophagy related 5) or Atg7 (autophagy related 7) in their epidermis to inhibit autophagy, we show that keratinocyte autophagy regulates wound healing in mice. Wounding induces the expression of autophagy genes in mouse skin. Epidermis-specific autophagy deficiency inhibits wound ...
WebNov 25, 2024 · ATG5 was identified as the only important harmful marker influencing survival of cervical cancer patients by univariate Cox regression (HR 1.7; 95% CI: …
WebOct 31, 2024 · Knockdown of ATG5 or ATG7 relieved autophagy and apoptosis induced by m-THPC-PDT in CRC cells It has been shown that ATG5 and ATG7, two major autophagy-related proteins, are important components ... csharp get stack traceWebMay 6, 2024 · Moreover, ATG5, ATG7 and ATG5 + ATG7 lead cells into more S phase, increase the number of S phase and inhibit apoptosis as well. ATG5, ATG7 and ATG5 + ATG7 regulate autophagy, ER stress, apoptosis and cell cycle through PERK signaling, a vital UPR branch pathway. ATG5 and ATG7 connect autophagy with ER stress through … csharpgl 下载Webcovalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin-E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). Directions for Use: CST recommends transfection with 100 nM Atg5 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the c sharp get type of objectWebJan 29, 2024 · CGTTP 3-72.9 Alternate Compliance Program (ACP) TTP 1-1 Chapter 1: Introduction Chapter 1: Introduction Introduction This chapter overviews the contents of … csharpglWebScale bar:50 μm. (K) Quantification of mitochondrial membrane potential (JC-1 aggregate/monomer ration). (L–M) Western blot analysis of PTGS2, NCOA4, FTH1, LC3, p-ULK1, Atg5 and Atg7 in H9C2 cells cotreated with PLG and OA/PA for 24 h (n = 3, *p < 0.05 vs. indicated group). (For interpretation of the references to colour in this figure ... csharp global usingWebAug 7, 2024 · Briefly, 1 mg of Dynabeads was conjugated to anti-FADD (Santa Cruz, sc-6036), anti-ATG5 (CST, 8540) antibody, goat IgG control (Santa Cruz, sc-2028) or rabbit IgG (Jackson ImmunoResearch, Ely, UK, 011-000-003) overnight at 4 °C. Dynabeads were washed in ice-cold NP-40 lysis buffer and combined with cell lysates on a rotor at 4 °C … csharp gotoWebMonoclonal Antibody for studying ATG5. Cited in 566 publications. Validated for Western Blotting, Immunoprecipitation. Available in 2 sizes. Highly … ea cricket ipl download