WebFlow cytometric analysis of Stat1 (pY701) expression in Unstimulated and Stimulated Human CD4+ T lymphocytes. Human peripheral blood leucocytes were either not stimulated (Unstimulated, dashed line histogram) or stimulated (Stimulated, solid line histogram) for 15 minutes with Phorbol 12-Myristate 13-Acetate (Sigma P-8139) as well as Recombinant … Phospho-flow has been applied to numerous areas of biology, including antigenic stimulation and microbial challenge, high-throughput and high-content drug discovery, as well as the characterization of signaling pathways in both normal and disease-altered immune responses. Fixing Your Cells For … See more When this is the case, I recommend that the researchers set up a phospho-specific flow cytometry, or phospho-flow, experiment. These types of experiments measure the … See more When performing and optimizing a phosphor-flow experiment, there are several things to consider. Here are 5 ways to optimize your phospho-flow experiment. See more Many protocols use 100% methanol for permeabilization following fixation. The advantage of this is that following methanol … See more When analyzing phoso-flow data, there are two things to keep in mind. First, the fluorescence intensities of each population will serve as a measure of the magnitude of the protein target’s phosphorylation … See more
Methodology Total Phosphorus, USEPA by Flow Injection …
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