Takara bio otsu japan
WebThe total RNA was isolated from kidney tissues using TRIzol Reagent (Thermo Fisher Scientific) according to the manufacturer’s manual. The reverse transcriptase reaction was performed to synthesize cDNA, and qRT-PCR assays were performed using SYBR Premix (Takara Bio, Inc., Otsu, Japan). Web13 apr 2024 · Then 1 μg of total RNA was reverse-transcribed into cDNA by using a PrimeScript RT reagent Kit (TAKARA BIO INC., Otsu, Japan) with six random primers (TAKARA BIO INC.).
Takara bio otsu japan
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Web14 ago 2024 · A reverse transcription reaction was performed using the PrimeScript RT reagent kit (Takara Bio, Otsu Japan). cDNA synthesized from 100 ng of total RNA was added to a PCR mixture containing SYBR Premix Ex Taq II (Takara Bio, Otsu Japan), one of the primer sets (OPRT [forward primer: 5’-TTG AAG ACC GGA AGT TTG CAG ATA … Web9 nov 2016 · TUNEL staining was performed with an in situ apoptosis detection kit (Takara-Bio Inc., Otsu, Japan). TUNEL-positive and -negative cells were counted in four randomly selected areas of a culture slide. The results are expressed as a ratio of TUNEL-positive cells to total cell number in each field.
WebTaKaRa lactate dehydrogenase ldh cytotoxicity detection kit Lactate Dehydrogenase Ldh Cytotoxicity Detection Kit, supplied by TaKaRa, used in various techniques. Bioz Stars … WebUse fluorescence quantitative PCR (qt-PCR) to detect mad2, The reagents used in the experiment include RNA extraction kits (Takara Bio, Inc., Otsu, Japan). And RNA reverse transcription kits (Takara Bio, Inc., Otsu, Japan). The length of mad2 mRNA is 163 bp, and GAPDH is used as an internal control. The DNA Ladder is 146 bp in length.
Web20 nov 2024 · The global headquarters of Takara Bio are in Otsu, Shiga, Japan. In 2005 the Company acquired Clontech Laboratories, Inc. (Mountain View, CA). Web1 mar 2024 · RNAiso Plus* (Code No.: 9109) 200 ml. * RNAiso Plus中含有强变性剂，应避免与皮肤、衣物等接触。. 若不小心接触到眼睛或皮肤时，请立即到医院进行处理。. 制品说明. 本制品可以从动物组织、植物材料、各种微生物、培养细胞等中提取Total RNA。. 样品在RNAiso Plus中能够充分 ...
Web2 1] Takara Bio Inc., Otsu, Shiga 520-2134, Japan [2]. 3 1] Department of Molecular and Medical Genetics, Oregon Health and Science University School of Medicine, Portland, …
Web28 mar 2014 · The amplification reaction was performed in a Takara PCR thermal cycler MP (Takara Bio, Otsu, Japan). These PCR experiments were performed three times. Amplified PCR products were separated using an automatic capillary electrophoresis system (QIAxcel Advanced, Qiagen) for detection of DNA fragments. how to date old budweiser bottlesWeb2 set 2024 · Total cellular RNA was reverse transcribed to make cDNA by PrimeScript™ RT Master Mix (Perfect Real Time) (TaKaRa Bio Inc., Otsu, Japan) and PrimeScript RT reagent (TaKaRa Bio Inc., Otsu, Japan) [44,45,46]. Relative fold induction was calculated with the 2 −ΔΔCt method. the miry clayWeb13 apr 2024 · Genomic DNA was isolated from tail samples of the offspring using standard methods. The edited region around exon 3 of the Slc35a3 gene was amplified using PCR with Ex Taq (TaKaRa Bio, Otsu, Japan) and the following primers: 5′- ACTGATTTTCCCCATCGTGT-3′ and 5′-CTTGCTTGAGGTAATGG-3′. how to date old canning jarsWebHuman renal proximal tubule epithelial cells were purchased from Takara Bio (Otsu, Japan). Renal epithelial cell growth kit and medium were also purchased from Takara Bio. Fetal bovine serum was purchased from Invitrogen. Six- or 96-well plates were purchased from Becton Dickinson (San Jose, CA, USA). how to date old glassWeb31 dic 2024 · Takara Bio is committed to working for the benefit of society through the use of biotechnology and Gene medicine developed from our research to enrich people's … how to date my marlin rifleWebRR064A. One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) 100 Rxns. USD $379.00. The One Step PrimeScript RT-PCR Kit (Perfect Real Time) is designed for one … how to date old pepsi glass bottlesWebPartnering with Takara Bio allows you to: Collaborate with our experts to develop reagent formulations that integrate into your workflow. Leverage our ISO-certified capabilities … how to date old soda bottles